Hello StartEngine community,

There is a few days left in our campaign and to invest in your health and the environment. With the recent train derailments and chemicals spills happening around the country, recent fungal and hepatitis outbreaks, and harmful algae blooms hitting Florida, there is continued proof that new ways of detection and cleanup are needed in our local communities and environment.

Jackman-Moose Fire Chief/Somerset County

Take this final opportunity to help jumpstart the positive change to help create better systems to detect outbreaks with AI before they spread and to cleanup the environment with natural rhamnolipid biosurfactants. Our applications have great potential and use cases against many of these types of scenarios. With the way the country is currently headed, these events will become more commonplace and the need for our applications will increase.

Invest and buy shares today in De3 Inc.

Learn more at de3.ai.

The use of cheap, synthetic, and petroleum-based chemicals is prevalent in essentially every industry around the world. These chemicals are toxic and cause harmful effects to people’s health and their environment, but they are still widely used as they are relatively inexpensive. Many long-term health and environmental effects are also disregarded until a crisis arises. Chemical suppliers care more about the number of sales of their products now versus the health of their customers and the surrounding areas later. We believe there doesn’t need to be a trade-off in effectiveness and toxicity versus price. That is where a natural and non-toxic chemical called rhamnolipid can be the solution.

Rhamnolipids are produced naturally in the environment. The bacteria pseudomonas aeruginosa is found everywhere from indoors to outdoors, from countertops in our kitchen to lakes, streams, and in soil. The chemical rhamnolipid is secreted by the bacteria to protect itself and survive. Harnessing these properties, we can produce rhamnolipid by a fermentation process similar to those used to produce beer or other fermented products. Unique strains from our library of bacteria are added to a fermentation tank and provided a nutrient source, such as an oil. Using unique processes, properly controlled conditions, and manufacturing trade secrets, low-cost rhamnolipids can be produced in industrial quantities. The rhamnolipids are extracted, processed to remove any residual bacteria, purified, and the resultant mixture diluted into a final product. The amount and mixture of rhamnolipid material in the final solution can be precisely controlled to specific ratios, mixtures, dilutions, and carriers. The rhamnolipid can be further refined to produce certain foaming characteristics and mixed according to specific applications, such as wetting agents for the agricultural industry. Our goal is to build a large-scale manufacturing facility to provide industrial-scale quantities of rhamnolipid biosurfactants to meet the demand of businesses worldwide.

Utilizing artificial intelligence, we can further increase the effectiveness of rhamnolipid. AI can provide recommendations and application-specific data to better refine the rhamnolipid processes and final product. If a hazard is detected in the field, the AI can recommend a solution based on that information. Time, effort, and funds can be saved in the research, development, testing, manufacturing, and application of rhamnolipid with the power of AI.

We have spent years in research, development, and testing of our rhamnolipid biosurfactants. Our rhamnolipid, RhamnoWash 10, is a naturally derived biosurfactant, consisting of lipid and rhamnose sugar molecules. On August 3, 2015, the U.S. Environmental Protection Agency (EPA) issued a letter to us regarding the listing of RhamnoWash 10™ as a Surface Washing Agent on the National Oil and Hazardous Substance Pollution Contingency Plan (NCP) Product Schedule maintained by the USEPA. In order to list RhamnoWash 10, technical product data was submitted to the USEPA pursuant to 40 CFR 300.915(b).

In 2004, The EPA issued a letter stating that rhamnolipid was accepted as a spray to kill pests on agricultural products in the United States. It also noted that rhamnolipid was also registered as “safe” to be ingested by humans at very low concentrations.

On February 24, 2014, we were granted approval by the Florida Department of Environmental Protection for rhamnolipid wastewater and soil washing applications. The letter indicates that the Division of Waste Management accepts Rhamnolipid Aqueous Solution for in situ bioremediation of petroleum hydrocarbons and metals in groundwater and soil. 

In 2021, we were granted permission to test and take samples in the Tampa Bay by the Florida Department of Environmental Protection.

In 2018, Helen Zhang, Professor at Memorial University, successfully tested our biosurfactants via an adsorption experiment for investigating cobalt removal from soil. 

Research Publication

"When applying rhamnolipid, by increasing the initial concentration of Co, the remaining Co in the solution increased considerably at any initial concentration of rhamnolipid. The high value range of surface tension of solutions (60-70 mN/m) shows that rhamnolipid has a high tendency of adsorption to the soil surface."

Rhamnolipid has a wide array of use cases such as:

• Eliminating viruses such as SARS2-CoV-2(Coronavirus)(1).
• Replacing synthetic chemicals in cosmetic applications (2).
• Agricultural/food industry applications (flavorings, plant nutrients, pesticides, fertilizers, etc.) (3).
• Environmental remediation (4). 
• Eliminating algae blooms (5). 
• Medical and pharmaceutical applications such as treating psoriasis, burn wound healing, drug delivery, etc. (6)(7)(8)

With many more applications spanning various industries. Its natural, non-toxic properties, and “green” manufacturing process with no harmful byproducts make it a unique substance that can replace the toxic synthetic alternatives widely used today. Utilizing AI, we can further unlock its capabilities, applications, and to further refine the manufacturing process. As we grow and start to produce rhamnolipid in industrial quantities from a large-scale manufacturing facility, we can begin to phase out and replace harmful alternatives.

Join our mission to replace harmful chemicals. Invest and buy shares today in De3 Inc.

Learn more at de3.ai.

AI is quickly changing the landscape in all facets of technology, businesses, the environment, health, communications, and many other sectors. With recent pandemics, contamination disasters, and general environment and water quality concerns, the need for AI to better serve our health has been pushed to the forefront.

De3 believes that we can be that transformational change by creating technologies and applications that can directly benefit people's health and the overall landscape of the current environment while utilizing artificial intelligence. We can be that preventative measure from future health and contamination disasters while leading the change from toxic chemicals to renewable and natural rhamnolipid biosurfactants with a wide array of applications. Data, analytics, predictive analysis, and knowledge from harnessing the computational power of AI is that change. 

Get a deeper dive into our AI system with Daniel Rojas as he explains some of our current and future AI capabilities. We are confident that with our technology and the increased demand for these types of applications, we will see healthy growth within the company while contributing to a healthier populace and environment.

Invest in your health and the environment around you by joining our mission and ambitious vision. Purchase your shares today.

Learn more at de3.ai and stay tuned for more updates, videos, live streams, and technology highlights.

Hello StartEngine community,

We are excited to finally go live on StartEngine and to interact with the community of investors/potential investors as we continue to grow and achieve our mission. This has been a long time coming and we are excited about our future opportunities.

Our journey began 20+ years ago when the founder, Keith Louis De Santo, first got into the rhamnolipid biosurfactant industry. He spent years on research and development, efficacy testing, EPA testing and registrations, intellectual property, partnerships with institutions (domestic and international), as a keynote speaker in biosurfactant symposiums, and many other opportunities and relationships. Given rhamnolipid's unique properties and use cases across a wide array of industries, there are endless ways to configure the end product to tackle specific needs, especially in the medical and remediation services sectors.

As artificial intelligence and machine learning began to grow during the late 2010s, the team realized that instead of manual testing of areas for potential contamination or outbreaks present and manual trial and error testing of the correct dilutions, carries, and ratios of specific rhamnolipid formulations for those scenarios, we could use the power of AI/machine learning to quickly identify and bolster our rhamnolipid applications. Testing can become faster and more automated, saving time, money, human error, and exposure to potentially hazardous material. When something is found, unique specific rhamnolipid application formulations that are most beneficial to the specific material can be recommended by the AI, leaving the guesswork and trial and error behind. AI can be used to optimize many industries and these applications are no exception.

When COVID-19 began to spread worldwide, we knew we had to find better ways of being a preventative measure and not a cure so we are not left unprepared when another widespread outbreak or contamination comes. It's not a matter of if, but when. The recent pandemic cemented our mission forward. That's what led us to where we are now. With rhamnolipid's natural and non-toxic applications coupled with AI to help detect micro-organisms and contamination, we believe we can replace toxic alternatives that are destroying our health and the environment and be the go-to preventative measure for future outbreaks. 

Invest today and share with your community so we tackle these problems together and we can all benefit from a cleaner and healthier environment.

More exciting updates to come!

-The DE3 team